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Use case: tracing electrode tracks
You can trace electrode tracks in Lasagna with Plugins > Annotation plugins > Add line plugin.
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Load the image stacks with
File>Load ingredient>New image stack. The whole stack is loaded into RAM. During loading the GUI is unresponsive. Just wait and it will load. -
You may load multiple channels and they will be automatically overlaid in different colours.
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Start the plugin.
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Select "Piecewise" from the drop-down selector box under Add/Remove.
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Scan through the stack to get a feel for where the track is.
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Use left-click to place the first point at the brain surface where the electrode entered. You may click on any of the axes, but the coronal plane may be easiest.
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Continue scrolling through the brain and adding points. New points are appended to the end of the line.
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You may go back an insert points between others by right-clicking on a point. It now becomes highlighted. You can highlight a different point, again with a right-click, if needed. You may cancel insert mode by right-clicking again near the highlighted point. The next point you add by left-clicking will be added between the highlighted point and the one before it.
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To remove a point select the "Remove" radio button. The highlighted point nearest the mouse will be removed when you left-click. Return to "Add" mode when done.